SpermFunc® Sperm DNA Fragmentation Test Kit

Name: SpermFunc® Sperm DNA Fragmentation Test Kit
Brand: BRED Life Science
SKU: BRED-002
Availability: InStock

$1,706.99

BRED Life Science’s SpermFunc® DNAf is a quick and easy-to-use test kit designed to evaluate the degree of sperm DNA fragmentation using sperm chromatin dispersion (SCD) protocols. This kit allows for controlled sperm denaturation and removal of proteins, resulting in easy identification of normal spermatozoa by their glowing ‘halos’ which are absent in sperm cells with damaged DNA. Test results can support the analysis of pathogenesis in cases of infertility, natural abortion, and inactive embryo development.

40 tests / box

Simple, quick, and reproducible results with optimized SCD protocols
Produces highly contrasted halo images that are easy to interpret
Results can be viewed under the microscope – no need for complex laboratory equipment
Tests can be completed within one hour
Reagents have a two-year shelf-life

SKU: BRED-002 Category: Consumables Tags: halo, SCD, sperm chromatin dispersion, stain Brand: BRED Life Science

Operation procedures video:

SpermFunc® DNAf allows for simple evaluation of sperm DNA fragmentation by an initial acid treatment that denatures DNA molecules in sperm cells with fragmented DNA, followed by removal of the nuclear proteins. Samples are then treated with Wright’s stain to produce distinctive halos in healthy spermatozoa, identifying degraded sperm cells by the absence of a halo. Sperm tails are preserved, providing clear identification of sperm cells for assessment.

In the figure above, panel 1 shows the sketch map of determination in sperm DNA fragmentation. A represents the smallest diameter of the sperm head, and B represents the thickness of the unilateral sperm halo. Generally, a small or absent halo indicates DNA fragmentation. Panels 2 and 3 demonstrate healthy sperm with no DNA fragmentation. Panels 4 and 5 demonstrate test results of sperm with high levels of DNA fragmentation.

Kits should be stored at 2-8° C in dark place.
Pre-coated slides may be stored at room temperature and should be kept away from moisture
Opened kits should be used within 58 days of opening
Unopened kits have a shelf life of 2 years when stored appropriately
Each kit contains:
- 20 pre-coated slides with two wells each – total of 40 tests
- 40 tubes of gel with low melting point – 0.14ml/tube
- 2 solutions – 100ml
- Wright’s stain – 20ml
- Wright’s buffer – 30ml
- SCD preservative Reagent – 15ml
- 45 glass slide covers
- Slide barrels